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Liberated Hb will initiate Fenton reactions to generate free radicals (Wagener et al., 2001)

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The solution should clear within a minute or two, yielding ~10 mg/mL total (5 mg/mL of each component)

Total proteins from lysed cells or patient tissue were extracted using RIPA lysis buffer and electrophoresed by SDS-PAGE and transferred onto PVDF membranes subsequently

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